Copy numbers and the number of clusters per cell for Swi4-mEos3.2, Mbp1-mEos3.2, Swi6-mEos3.2, and Whi5-mEos3.2 increase with cell size. (A–D) Total number of Swi4-, Mbp1-, Swi6-, and Whi5-mEos3.2 molecules, respectively, in each nucleus as a function of nuclear size, a proxy for cell size. Each point represents an individual nucleus. (E–H) Number of Swi4-, Mbp1-, Swi6-, and Whi5-mEos3.2 clusters per nucleus as a function of cell size. (I–L) Average number of Swi4-, Mbp1-, Swi6-, and Whi5-mEos3.2 molecules per cluster for each nucleus as a function of cell size. Moving from left to right, panels correspond to Swi4-mEos3.2 (green, 370 cells), Mbp1-mEos3.2 (red, 536 cells), Swi6-mEos3.2 (blue, 222 cells) and Whi5-mEos3.2 (pink, 155 cells). Nuclear size is given in pixels. To the left of the dashed lines at 20,000 pixels, the majority of cells are in G1 phase as determined by the presence of Whi5-mEos3.2 in the nucleus (D, H, and L).