Pretreatment of teNK cells with FDA-approved DNMT inhibitors neutralizes the teNK cell phenotype. (A) Heat map of z-scores of gene expression by hNK cells and teNK cells of genes related to DNMTs. Hierarchical clustering was used to order the genes. (B) Schema of pretreatment of teNK cells before coculture with tumor clusters. (C) Normalized colony count from DMSO control or DNMT inhibitor pretreated teNK cell–MMTV-PyMT tumor cluster coculture assays versus monoculture controls. Mean with SEM is represented. n.s., not significant; *, P < 0.05; **, P < 0.01; ****, P < 0.0001 by Mann–Whitney U test. (D and E) Normalized colony count from DMSO or DNMT inhibitor pretreated teNK cells and antibody treated monoculture control assays and teNK cell–tumor cluster coculture assays. Mean with SEM is represented. *, P < 0.05; **, P < 0.01 by Mann–Whitney U test.