RNA-seq analysis of hNK cells and teNK cells reveals differences in identity and biological processes. Receptor–ligand analysis of hNK cells and K14⁺ or K14⁻ cells reveals interactions between NK cells and cancer cells. Treatment with DNMT inhibitors alter gene expression of inhibitory receptors. (A) Schema for RNA-seq analysis of hNK cells and teNK cells. (B) Gene ontology enrichment analysis in “biological process” category for genes differentially expressed between hNK and teNK cells. 30 categories with the lowest P value associated with up- or down-regulated teNK cells are displayed. (C and C′) Network representation of total receptor–ligand pairs between hNK cells (C) or teNK cells (C′) and K14⁺ or K14⁻ cells as identified by the databases included in the iTalk algorithm. (D) Relationship map of receptor–ligand pairs of hNK cells and K14⁺ or K14⁻ cells as identified by the databases included in the iTalk algorithm. (E) Treatment of teNK cells with azacitidine or decitabine alters gene expression of TIGIT and KLRG1 by qPCR. Because of logistical constraints during the COVID-19 pandemic, the assays were able to be conducted only twice and once, respectively.