Actin-dependent Pav function is required for cell wound repair. (A–D) Actin dynamics (sGMCA or sStMCA) during cell wound repair in NC4–6 staged embryos: control (injection buffer alone; A), Pav^DEAD (B), colchicine injection (C), Pav^DEAD with colchicine injection (D). (A’–D’) xy kymograph across the wound area depicted in A–D, respectively. (E) Quantification of the wound area over time for control (injection buffer alone; n = 10), Pav^DEAD (n = 10), colchicine injection (n = 10), and Pav^DEAD with colchicine injection (n = 10). (F and G) Quantification of wound expansion time (F) and wound closure speed (G), control (buffer injection), Pav^DEAD, colchicine injection, and Pav^DEAD with colchicine injection (n = 10 for each condition). Time after wounding is indicated. Scale bar: 20 µm. Error bars represent ± SEM. Unpaired Student’s t tests were performed in F and G.