Actin-dependent Pav function is required for oogenesis. (A) Quantification of the percentage of egg chambers exhibiting actin defects. (B–I) Posterior of stage 7 egg chambers, stained for F-actin, in wild type (B), wimp/+ (C), reduced pav^B200 (D), reduced pav⁹⁶³ (E), tum^RNAi(1) (F), tum^RNAi(2) (G), and tum^RNAi(2) : wimp/+ (H): Pav^DEAD (I). Arrows indicate cortical actin projections from oocyte (ooc) cortex. Arrowhead indicates actin aggregation in the oocyte. (J–Q) Cortical actin mesh structure in wild type (J), wimp/+ (K), reduced pav^B200 (L), reduced pav⁹⁶³ (M), tum^RNAi(1) (N), tum^RNAi(2) (O), and tum^RNAi(2) : wimp/+ (P): Pav^DEAD (Q). (R–Y) Quantification of actin mesh structure with GLCM texture analysis for wild type (R), wimp/+ (S), reduced pav^B200 (T), reduced pav⁹⁶³ (U), tum^RNAi(1) (V), tum^RNAi(2) (W), and tum^RNAi(2) : wimp/+ (X): Pav^DEAD (Y). (Z–GG’) Single time point (Z–GG) and 30-min time point projections (Z’–GG’) from time-lapse movies of stage 7 egg oocytes in wild type (Z and Z’), wimp/+ (AA and AA’), reduced pav^B200 (BB and BB’), reduced pav⁹⁶³ (CC and CC’), tum^RNAi(1) (DD and DD’), tum^RNAi(2) (EE and EE’), tum^RNAi(2) : wimp/+ (FF and FF’), and Pav^DEAD (GG and GG’). (HH) Quantification of the percentage of egg chambers exhibiting premature ooplasmic streaming. n for each genotype is indicated on bar plots; Fisher’s exact test was performed in A and HH, and unpaired Student’s t test was performed in R–Y; ns, not significant. ***, P < 0.0001. Scale bars: 20 µm in Z–GG’ and 10 µm in B–Q.