Pav and Tum are required for the organization of actin in outer ring canals. (A–B”) Oblique view of the ring canal connecting two nurse cells in the stage 7 egg chambers, stained for anti-Pav and F-actin in wild type (A–A”) and Pav RNAi mutant (B–B”). (C–J) Oblique view of the ring canal connecting two nurse cells in stage 7 egg chambers, stained for F-actin, in wild type (C), wimp/+ (D), reduced pav^B200 (E), reduced pav⁹⁶³ (F), tum^RNAi(1) (G), tum^RNAi(2) (H), and tum^RNAi(2) : wimp/+ (I): Pav^DEAD (J). Brackets indicate outer ring canal. (K–R) Cross section of the ring canal connecting an oocyte and a nurse cell in stage 7 egg chamber, stained for F-actin, in wild type (K), wimp/+ (L), reduced pav^B200 (M), reduced pav⁹⁶³ (N), tum^RNAi(1) (O), tum^RNAi(2) (P), and tum^RNAi(2) : wimp/+ (Q): Pav^DEAD (R). Arrow indicates inner ring canal; arrowhead indicates outer ring canal. Scale bars: 10 μm.