Pav and Tum also exhibit independent localizations in the ovary. (A–A’”) GFP-Tum–expressing stage 7 egg chamber stained for Tum (anti-GFP), Pav (anti-Pav), and F-actin (phalloidin). Scale bar: 50 µm. (B–C’”) Oblique view of the ring canal connecting two nurse cells (B–B’”) and cross section of the ring canal connecting an oocyte and a nurse cell (C–C’”) in the stage 7 egg chambers. Brackets indicate outer actin ring canals. Scale bar: 10 µm. (D–D”) Protein distribution at the GFP-Tum–expressing oocyte cortex in the stage 7 egg chamber, stained for Tum (anti-GFP), Pav (anti-Pav), and F-actin (phalloidin). Scale bar: 10 µm. (E–F”) High-magnification views from two different boxed areas in D”. Scale bar: 1 µm.