Pav and CapuFH2 bundle and cross-link actin/MTs in a concentration-dependent manner. (A and A’) Stabilized actin and MTs incubated with Tum. (B–J”) Stabilized actin and/or MTs were incubated with different concentrations of Pav (B–E”) and CapuFH2 (F–J”). Protein concentration is indicated. (K–L””) Stabilized actin and MTs were incubated with CapuFH2 to bundle actin/MTs, followed by the addition of GFP-Pav (K–K””) or GFP-Pav^DEAD (L–L’”). Scale bar: 30 µm. Final protein concentrations for bundling assays: CapuFH2, 500 nM; GFP-Pav, 3 nM; and GFP-Pav^DEAD, 3 nM.