Pav bundles F-actin/MTs and cross-links them, even in the presence of Tum. (A) The scheme of bundling/cross-linking assays. (B–H) Stabilized actin and/or MTs were incubated with no protein (B and B’), full-length Pav protein, or full-length Pav/Tum proteins (C–H). Final protein concentrations for bundling assays: Pav, 200 nM; Pav + Tum, 200 nM Pav. Scale bar: 30 µm.