Spatial and temporal patterns of Rho family GTPases are not altered in reduced pav mutant backgrounds. (A–C”) Localization of Rho family GTPases along with an actin reporter (sGMCA) in NC4–6 staged control embryos (wimp/+): ChFP-Rho1 (A–A”), ChFP-Rac1 (B–B”), and ChFP-Cdc42 (C–C”). (D–F”) Localization of Rho family GTPases along with an actin reporter (sGMCA) in NC4–6 staged reduced pav embryos: ChFP-Rho1 (D–D”), ChFP-Rac1 (E–E”), and ChFP-Cdc42 (F–F”). (G) Quantification of the area under the curve in each fluorescence intensity profile from 10–12 individual embryos. ns, not significant. Unpaired Student’s t tests were performed in G. n and time after wounding are indicated. Scale bar: 20 µm. Error bars represent ±SEM.