Pav and Tum mutants exhibit distinct phenotypes. (A) Western blot analysis of Pav protein levels (100 kD) in lysates from wild type (WT), reduced pav^B200, and reduced pav⁹⁶³. (B) Western blot analysis of Tum protein levels (70 kD) in lysates from WT and tum^RNAi(1). (C–I’) Actin dynamics (sGMCA or sChMCA) during cell wound repair in NC4–6 staged embryos: control (wimp/+; C), reduced pav⁹⁶³ (wimp^+/+pav⁹⁶³; D), reduced pav^B200 (wimp^+/+pav^B200; E), control (GAL4 driver alone; F), tum^RNAi(1) (G), tum^RNAi(2) (H), tum^RNAi(2) : wimp/+ (I). (C’–I’) xy kymograph across the wound area depicted in C–I, respectively. (J) Quantification of the wound area over time for control (wimp/+; n = 12), reduced pav⁹⁶³ (n = 12), and reduced pav^B200 (n = 10). (K) Quantification of the average intensity within the actin ring over time relative to UW for control (wimp/+; n = 5), reduced pav⁹⁶³ (n = 5), and reduced pav^B200 (n = 5). (L) Quantification of the wound area over time for control (GAL4 driver alone; n = 14), tum^RNAi(1) (n = 11), tum^RNAi(2) (n = 13), and tum^RNAi(2) : wimp/+ (n = 12). (M) Quantification of the average intensity within the actin ring over time relative to UW for control (GAL4 driver alone; n = 5), tum^RNAi(1) (n = 5), tum^RNAi(2) (n = 5), and tum^RNAi(2) : wimp/+ (n = 5). (N–Q) Quantification of wound closure speed (N), wound expansion time (P), and actin ring width (O and Q) for control (wimp/+), reduced pav⁹⁶³, reduced pav^B200, control (GAL4 driver alone), tum^RNAi(1), tum^RNAi(2), and tum^RNAi(2) : wimp/+. (R–R’) Confocal xy projection images from a NC4–6 staged Drosophila embryo coexpressing an actin marker (sChMCA) and GFP-Pav in a tum^RNAi(2) background. (S) Quantification of the area under the curve in each fluorescence intensity profile form control (GAL4 driver only; n = 14) and tum^RNAi(2) (n = 15). ns, not significant. (T–T’) Confocal xy projection images from a NC4–6 staged Drosophila embryo coexpressing an actin marker (sStMCA) and sfGFP-Tum in a reduced pav⁹⁶³ background. (U) Quantification of the area under the curve in each fluorescence intensity profile form control (wimp/+; n = 11) and reduced pav⁹⁶³ (n = 12). Time after wounding is indicated. Scale bar: 20 µm. Error bars represent ±SEM. Unpaired Student’s t tests were performed in N–Q, S, and U).