Pbl, Tum, and Pav exhibit distinct localization patterns in cell wound repair. (A) Confocal xy projection image from a laser-wounded NC4–6 staged Drosophila embryo expressing an actin reporter (sGMCA). Schematic diagram summarizing the localization patterns of actin, Pbl, Tum, and Pav at the wound edge. (B–B”) Confocal xy projection images from NC4–6 staged Drosophila embryo coexpressing an actin reporter (sChMCA) and Pbl-eGFP. (C) Fluorescence intensity (arbitrary units) profiles across the wound area in B”. (D–D”) Confocal xy projection images from a NC4–6 staged Drosophila embryo coexpressing an actin reporter (sChMCA) and sfGFP-Tum. (E) Fluorescence intensity (arbitrary units) profiles across the wound area in D”. (F–F”) Confocal xy projection images from an NC4–6 staged Drosophila embryo coexpressing an actin marker (sChMCA) and GFP-Pav. (G) Fluorescence intensity (arbitrary units) profiles across the wound area in F”. (H–H”) Confocal xy projection images from a NC4–6 staged Drosophila embryo coexpressing sfGFP-Tum and Ch-Pav. (I) Fluorescence intensity (arbitrary units) profiles across the wound area in H”. (J) Western blot analysis of Pav protein levels in wild type and GFP-Pav–expressing embryos under our imaging conditions (*, endogenous Pav; **, GFP-Pav). (K) qPCR analysis of Tum expression in wild-type and GFP-Tum expressing embryos under our imaging conditions. Error bars represent ± SEM. (L–M””) GFP-Tum–expressing embryos stained for Tum (anti-GFP), Pav (anti-Pav), and F-actin (phalloidin). Scale bar: 20 µm. Time after wounding is indicated. In fluorescence intensity profiles, the line represents the averaged fluorescent intensity, and the gray area is the 95% confidence interval from a 10-pixel section of the image shown (B”, D”, F”, or H”).