Nuclear speckle localization of MFAP1. (A) Immunofluorescence images of U2OS cells after SC35 and MFAP1 coimmunostaining following treatment with either DMSO or DRB for 2 h. Cells were fixed with 100% methanol. (B) Representative immunofluorescence image of a mitotic U2OS cell after SC35 and MFAP1 coimmunostaining following PFA fixation. DNA (blue) was stained with DAPI. Scale bars: 10 µm.