Effects of SNX9 knockdown on CCP dynamics. (A) Single frame from TIRFM video (7.5 min/video, see Video 5) and corresponding kymograph from region indicated by the gray line of ARPE/HPV cells expressing eGFP-CLCa. (B) Effect of SNX9 knockdown on the initiation rates of bona fide CCPs and subthreshold CCSs. (C) Mean clathrin fluorescence intensity traces of lifetime cohorts of analyzed clathrin structures. Growth phase is indicated by blue arrows. (D) Mean slopes of the fluorescence intensity cohorts shown in C measured during the growth phase from t = 3 s to t = 8 s. Each dot represents mean slope of a cohort. (E) Maximum fluorescence intensity (I_max) distribution of CCPs in control and SNX9 knockdown cells. (F) Medium lifetime (LT_50) of CCPs in control and SNX9 knockdown cells. Data presented were obtained from a single experiment that is representative of two independent repeats. n = 24 videos for each condition. Number of analyzed tracks: 106,310 for control and 77,932 for +TDD. Number of dynamic tracks analyzed: 294,804 for siControl and 152,013 for siSNX9. Error bars are SD. *, P ≤ 0.05; ***, P ≤ 0.001.