Figure S3.
Effect of TDD expression on endogenous protein expression, clathrin-coat stability, and AP1-mediated clathrin trafficking from Golgi.(A) ARPE/HPV cells expressing eGFP-CLCa cells were treated with or without TDD overexpression. TDD expression did not alter the expression levels of endogenous clathrin or AP2. (B) Representative TIRF images of CCPs in live cells or isolated TCVs. Scale bars = 5 µm. (C) Representative EM images showing the collapsed coats typical of isolated TCVs. Scale bars = 200 nm. (D) Immunostaining of HA-TDD in ARPE/HPV eGFP-CLCa cells. Scale bar = 10 µm. (E–G) ARPE cells were treated with or without TDD overexpression. Immunostaining of AP1 γ subunit (E) and GM-130 cis-Golgi marker (F) were imaged with wide-field microscopy. Scale bars = 10 µm. (G) Representative EM images of Golgi apparatus. Scale bars = 500 nm.

Effect of TDD expression on endogenous protein expression, clathrin-coat stability, and AP1-mediated clathrin trafficking from Golgi. (A) ARPE/HPV cells expressing eGFP-CLCa cells were treated with or without TDD overexpression. TDD expression did not alter the expression levels of endogenous clathrin or AP2. (B) Representative TIRF images of CCPs in live cells or isolated TCVs. Scale bars = 5 µm. (C) Representative EM images showing the collapsed coats typical of isolated TCVs. Scale bars = 200 nm. (D) Immunostaining of HA-TDD in ARPE/HPV eGFP-CLCa cells. Scale bar = 10 µm. (E–G) ARPE cells were treated with or without TDD overexpression. Immunostaining of AP1 γ subunit (E) and GM-130 cis-Golgi marker (F) were imaged with wide-field microscopy. Scale bars = 10 µm. (G) Representative EM images of Golgi apparatus. Scale bars = 500 nm.

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