Figure S2.
Clathrin and AP2 exchange on plasma membrane is inhibited by TDD expression. (A–C) FRAP was conducted at 37°C in ARPE/HPV eGFP-CLCa cells with or without TDD overexpression using a confocal microscope. (A) Representative time-lapse FRAP images. (B) Average fluorescent intensity traces of the photobleached area (dark square at t = 0 s). (C) Time required for 50% fluorescence recovery. (D–F) FRAP was conducted at 37°C in ARPE α-eGFP-AP2 cells with or without TDD overexpression using a confocal microscope. (D) Representative time-lapse FRAP images. (E) Average fluorescent intensity traces of the photobleached area (dark square at t = 0 s). (F) Time required for 50% fluorescence recovery. Error bars are SD from n = 8 experiments. Scale bars = 3 µm. The lines in these scatter dot plots represent the means with SD. **, P ≤ 0.01.

Clathrin and AP2 exchange on plasma membrane is inhibited by TDD expression. (A–C) FRAP was conducted at 37°C in ARPE/HPV eGFP-CLCa cells with or without TDD overexpression using a confocal microscope. (A) Representative time-lapse FRAP images. (B) Average fluorescent intensity traces of the photobleached area (dark square at t = 0 s). (C) Time required for 50% fluorescence recovery. (D–F) FRAP was conducted at 37°C in ARPE α-eGFP-AP2 cells with or without TDD overexpression using a confocal microscope. (D) Representative time-lapse FRAP images. (E) Average fluorescent intensity traces of the photobleached area (dark square at t = 0 s). (F) Time required for 50% fluorescence recovery. Error bars are SD from n = 8 experiments. Scale bars = 3 µm. The lines in these scatter dot plots represent the means with SD. **, P ≤ 0.01.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal