SARM1 KO does not prevent cell death induced by dimerization of MLKL.ND. (A) Representative bright-field and fluorescent images at 0 or 24 h after BB addition to WT or SARM1 KO DRG neurons that both express MLKL.ND and cytosolic GFP. Cells are stained with EthD (red) and imaged for GFP (green) to identify dead cells (EthD⁺ and GFP⁻). Scale bar, 50 µm. (B) Quantification of cell death of WT and SARM1 KO DRG neurons expressing MLKL.ND with two different markers for cell death 24 h after BB application. Left: EthD, n = 5; PI, n = 4; Data represent the mean ± SEM; for EthD, two-tailed unpaired t test, P = 0.34; for PI, two-tailed unpaired t test, P = 0.23.