Analysis of VF cdka;1/− cycb3;1/− mutant and functionality of the CYCB3;1:GFP reporter construct. (A) 9-d-old seedlings grown on plates with DMSO and 100, 150, and 200 nM oryzalin (upper panel). Mutants in the spindle assembly checkpoint component MAD1 are used as a positive control for hypersensitivity to oryzalin. Scale bar, 2 cm. Root length on plates with and without oryzalin (lower panel). The mean (± SD) of >20 seedlings per indicated genotype is shown. Level of significance (*, P < 0.05; **, P < 0.01; ***, P < 0.001***) determined by one-way ANOVA followed by Tukey’s test. (B) The main stem and siliques of VF cdka;1/− cycb3;1/− mutant in comparison to WT. Scale bar, 3 cm. (C) Siliques of WT, cycb3;1/−, and VF cdka;1/− cycb3;1/− double mutant, which show seed abortion highlighted by red asterisks. Scale bar, 1 mm. (D) Quantification of seed abortion in at least six siliques for each genotype. The percentage of viable seeds is represented by blue bars, and red bars indicate the percentage of aborted seeds from C. (E) Peterson staining of anthers for WT, cycb3;1/−, and VF cdka;1/− cycb3;1/−. Scale bar, 20 µm. (F) Quantification of pollen viability from at least six flower buds for each genotype. Blue bars indicate the percentage of viable pollen, and red bars give the percentage of aborted pollen from E.