Figure 2.
Analysis of meiotic defects in VF and VF cdkd;3 double mutant combinations.(A) Repartition of meiotic stages within one single flower bud undergoing meiosis from metaphase I to telophase II/tetrad stage in WT (n = 319), VF cdka;1/− (n = 379), VF cdka;1/− cdkd;3/+ (n = 275), and VF cdka;1/− cdkd;3/− (n = 253). (B) Chromosome spreads of male meiocytes in VF cdka;1 and VF cdka;1 cdkd;3 double mutants. Red arrows indicate univalents/fragments in metaphase I, white arrows pinpoint chromosome bridges in anaphase I and/or telophase II, blue arrows highlight micronuclei in interkinesis, and orange arrows point to premature cell wall formation in interkinesis. Scale bar, 10 µm. (C) Quantification of meiotic defects observed in VF cdka;1 cdkd;3 double mutants versus WT given in percentage of meiocytes of one genotype that show the respective feature. The numbers under every column indicate the meiocytes found per stage.

Analysis of meiotic defects in VF and VF cdkd;3 double mutant combinations. (A) Repartition of meiotic stages within one single flower bud undergoing meiosis from metaphase I to telophase II/tetrad stage in WT (n = 319), VF cdka;1/− (n = 379), VF cdka;1/− cdkd;3/+ (n = 275), and VF cdka;1/− cdkd;3/− (n = 253). (B) Chromosome spreads of male meiocytes in VF cdka;1 and VF cdka;1 cdkd;3 double mutants. Red arrows indicate univalents/fragments in metaphase I, white arrows pinpoint chromosome bridges in anaphase I and/or telophase II, blue arrows highlight micronuclei in interkinesis, and orange arrows point to premature cell wall formation in interkinesis. Scale bar, 10 µm. (C) Quantification of meiotic defects observed in VF cdka;1 cdkd;3 double mutants versus WT given in percentage of meiocytes of one genotype that show the respective feature. The numbers under every column indicate the meiocytes found per stage.

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