Figure 1.
Analysis of meiotic defects in cdkd mutants.(A) Chromosome spread analysis of male meiocytes in WT versus single cdkd;3 mutants and two different double cdkd;1 cdkd;3 mutant combinations during pachytene, metaphase I, interkinesis, metaphase II, and telophase II. Red arrows indicate univalents in metaphase I, and white arrows designate chromosome bridges in telophase II. (B) Close-up of chromosomes with missing chiasmata in metaphase I of cdkd;1/+ cdkd;3/−. Red circles highlight univalents, and blue circles, bivalents. (C–E) Unbalanced chromosome pools in interkinesis (C) and metaphase II (D), and a chromosome bridge in telophase II (E) in cdkd;1/+ cdkd;3/− double mutants. In the last row, quantification of meiotic defects observed in cdkd;3/− (n = 66), cdkd;1/− cdkd;3/+ (n = 50), and cdkd;1/+ cdkd;3/− (n = 58) versus WT (n = 115). The numbers under every column indicate the meiocytes found per stage. Scale bar, 10 µm.

Analysis of meiotic defects in cdkd mutants. (A) Chromosome spread analysis of male meiocytes in WT versus single cdkd;3 mutants and two different double cdkd;1 cdkd;3 mutant combinations during pachytene, metaphase I, interkinesis, metaphase II, and telophase II. Red arrows indicate univalents in metaphase I, and white arrows designate chromosome bridges in telophase II. (B) Close-up of chromosomes with missing chiasmata in metaphase I of cdkd;1/+ cdkd;3/−. Red circles highlight univalents, and blue circles, bivalents. (C–E) Unbalanced chromosome pools in interkinesis (C) and metaphase II (D), and a chromosome bridge in telophase II (E) in cdkd;1/+ cdkd;3/− double mutants. In the last row, quantification of meiotic defects observed in cdkd;3/− (n = 66), cdkd;1/− cdkd;3/+ (n = 50), and cdkd;1/+ cdkd;3/− (n = 58) versus WT (n = 115). The numbers under every column indicate the meiocytes found per stage. Scale bar, 10 µm.

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