ERdj8 depletion causes a defect in the enwrapping of larger mitochondria. (A–C) HeLa cells expressing mCherry-Parkin were treated with siRNA (siERdj8no1, siERdj8no2, and control). The cells were treated with CCCP or DMSO for 20 h, immunostained with Cox-II antibody, and imaged on a DeltaVision system. Scale bar, 10 µm. (B) The lysates were subjected to immunoblotting. (C) The average and SEM of three independent experiments of the ratio of Cox-II to GAPDH band intensity. *, P < 0.05. (D and E)C. elegans expressing myo-3::GFP(mito) were treated with or without dnj-8 and/or atg-5 RNAi. Embryos around the 1.5-fold stage were observed on an LSM700. Scale bar, 10 µm. (E) Total GFP signal intensity per worm. The average of 129 worms is shown. ***, P < 0.005. Results are reported as means ± SEM. (F and G) GFP::LGG-1 (green) and paternal mitochondria (mt; HSP-6::mCherry; red) in mock or dnj-8 (RNAi)-treated 1 cell–stage C. elegans embryos at the pronuclear expansion stage (F) or at the 64-cell stage (G) were observed on an FV1000. Fluorescence images merged with DIC images are shown. Insets, enlargements of framed regions. Scale bar, 10 µm.