Figure 1.
MT network and acetylated tubulin localize at the NMJ.(A, B, D, and E) Cross sections (A, D) and isolated fibers (B, E) of TA muscle from 2-mo-old WT mice in the presence (D, E) or absence (A, B) of taxol were double-stained with an antibody against α-tubulin to label MT network (A and B; β-tub, in red) or against acetylated tubulin to label stable MT (D and E; ac-tub, in red) and with α-BTX–A488 (in green) to label NMJs. (C and F) The fluorescence intensity of each staining was plotted as a function of the distance (based on the blue line scans in B and E, respectively). Arrowheads and arrows show enrichment of the MT network surrounding NMJs. Dashed lines indicate edges of cells. (A, B, D, and E) Scale bars: 25 µm; inset magnifications (boxes 1, 3, 4, and 6): 10 µm; (boxes 2 and 5): 2 µm. Fluo., fluorescence; β-tub, β-tubulin; ac-tub, acetylated tubulin.

MT network and acetylated tubulin localize at the NMJ. (A, B, D, and E) Cross sections (A, D) and isolated fibers (B, E) of TA muscle from 2-mo-old WT mice in the presence (D, E) or absence (A, B) of taxol were double-stained with an antibody against α-tubulin to label MT network (A and B; β-tub, in red) or against acetylated tubulin to label stable MT (D and E; ac-tub, in red) and with α-BTX–A488 (in green) to label NMJs. (C and F) The fluorescence intensity of each staining was plotted as a function of the distance (based on the blue line scans in B and E, respectively). Arrowheads and arrows show enrichment of the MT network surrounding NMJs. Dashed lines indicate edges of cells. (A, B, D, and E) Scale bars: 25 µm; inset magnifications (boxes 1, 3, 4, and 6): 10 µm; (boxes 2 and 5): 2 µm. Fluo., fluorescence; β-tub, β-tubulin; ac-tub, acetylated tubulin.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal