ET of reovirus egress machinery . HBMECs were adsorbed with reovirus T1L M1-P208S at an MOI of 1 PFU/cell and processed at 18 hpi by high-pressure freezing, freeze substitution, semithick sectioning, and TEM. (A and B) Single-tilt tomography. 3D reconstruction and computational tomography slices show details of the connection between the components of the reovirus egress machinery. The dashed box marks the specific point of membrane connection between an SO and a VI (A) and an MC linked to an SO through a channel (B). mi, mitochondrion. Scale bars, 200 nm.