Figure 8.
Superresolution live cell microscopy of lysosomes in reovirus-infected cells. HeLa cells were transfected with a σ3-GFP plasmid for 24 h and adsorbed with reovirus T1L M1-P208S at an MOI of 10 PFUs/cell. At 16 hpi, cells were imaged using TIRF microscopy to visualize basal reovirus egress zones. (A) TIRF microscopy images showing lysosomes stained with LysoTracker (red) and σ3 visualized with the GFP fusion protein (green). Arrows indicate the association of lysosomes and reovirus σ3 over time. (B) Fluorescence signal intensity corresponding to lysosomes and reovirus was quantified in a representative area using LAS X software (Leica Microsystems). The graphs confirm the colocalization of signals associated with LysoTracker and σ3-GFP. Another example of a reovirus-infected cell is shown Videos 7 and 8. A time-lapse recording of uninfected cells is shown in Video 9.

Superresolution live cell microscopy of lysosomes in reovirus-infected cells . HeLa cells were transfected with a σ3-GFP plasmid for 24 h and adsorbed with reovirus T1L M1-P208S at an MOI of 10 PFUs/cell. At 16 hpi, cells were imaged using TIRF microscopy to visualize basal reovirus egress zones. (A) TIRF microscopy images showing lysosomes stained with LysoTracker (red) and σ3 visualized with the GFP fusion protein (green). Arrows indicate the association of lysosomes and reovirus σ3 over time. (B) Fluorescence signal intensity corresponding to lysosomes and reovirus was quantified in a representative area using LAS X software (Leica Microsystems). The graphs confirm the colocalization of signals associated with LysoTracker and σ3-GFP. Another example of a reovirus-infected cell is shown Videos 7 and 8. A time-lapse recording of uninfected cells is shown in Video 9.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal