EPLIN requires LUZP1 to rescue cytochalasin D–induced ciliation. (A) MyosinVa fluorescence intensity at the centrosome of RPE-1 cells transfected with control siRNAs or siRNAs directed to LUZP1 or EPLIN. The graph shows data pooled from three independent experiments. The bar indicates average intensity. ***, P < 0.01 (Student’s two-tailedt test). (B) Representative images of control and LUZP1 and EPLIN depleted RPE-1 cells stained for MyosinVa and PCNT (centrosome marker). (C–E) WT and transgenic RPE-1 cells were transfected for 72 h with control siRNA or siRNAs targeting LUZP or EPLIN, as indicated. During the last 16 h of transfection, cells were treated either with DMSO (control) or 50 nM cytochalasin D. Cells were stained for γ-tubulin (centrosome and basal body marker) and ARL13B (ciliary marker). DNA was stained with DAPI. (F) Bar graph shows the mean percentage of ciliated cells (n > 200 cells per sample, three independent experiments) in the indicated cell lines transfected with control, LUZPI, or EPLIN siRNAs and treated with DMSO or 50 nM cytochalasin D. Error bars indicate SD. **, P < 0.01; ***, P < 0.001 (Student’s two-tailed t test).