LUZP1 and EPLIN subcellular localization analyses. (A) LUZP1 localizes to the centrosome and basal body. RPE-1 cells stably expressing GFP-LUZP1 were stained for GFP, glutamylated tubulin (centrosome and ciliary marker), ARL13B (cilia marker), and CEP290 (centriolar satellite).(B) LUZP1 localizes to actin filaments and the midbody (indicated by arrowhead). RPE-1 cells stably expressing GFP-LUZP1 were stained for GFP. The actin cytoskeleton was stained with fluorophore-conjugated phalloidin, and DNA was stained with DAPI.(C) LUZP1 localizes to the proximal domain of the centriole/basal body. RPE-1 cells stably expressing GFP-LUZP1 were stained for GFP, glutamylated tubulin (centrosome and ciliary marker), and CEP290 (centrosome and transition zone marker; top panel), CEP164 (distal appendage/transition fiber marker), or CEP170 (subdistal appendage marker).(D) IF analysis of RPE-1 GFP-LUZP1 stable cells treated with DMSO or nocodazole. Cells were stained for GFP, PCNT, and α-tubulin. Arrowheads indicate the centrosome. (E) IF analysis of HEK293 FLAG-BirA*-LUZP1 stable/inducible cells without treatment, treated with tetracycline only, or treated with tetracycline and biotin. Cells were stained with antibodies against FLAG and PCNT. Biotinylated proteins were detected with fluorophore-conjugated streptavidin and DNA with DAPI. (F) IF analysis of EPLIN subcellular localization. RPE-1 cells were stained with an antibody against EPLIN and phalloidin (top panel). RPE-1 cells stably expressing GFP-EPLINα or GFP-EPLINβ were stained with an antibody against GFP and phalloidin (middle and bottom panels). DNA was stained with DAPI. (G) RPE-1 cells stably expressing GFP-EPLINα or GFP-EPLINβ were stained with antibodies against GFP, ARL13B (cilia marker), and glutamylated tubulin (centriole and cilia marker).