Subcellular localization of LUZP1, a novel centrosome/basal body and actin-associated protein. (A) Schematic representation of human LUZP1, a 1,796-aa protein containing three leucine-zipper motifs and a LCD1 domain at its N-terminal region. Represented are also two truncation mutants. (B) LUZP1 localizes to the centrosome. RPE-1 cells were stained for LUZP1 and centrosome markers PCNT (pericentriolar material marker) and glutamylated tubulin (centriole marker). (C) LUZP1 localizes to the basal body. RPE-1 cells stably expressing ARL13B-GFP were stained for GFP, LUZP1, CEP164 (distal appendage/transition fiber marker), and glutamylated tubulin (centrosome and ciliary marker). (D) LUZP1 localizes to actin filaments and the midbody (indicated by the arrowhead). RPE-1 cells were stained using an antibody against LUZP1. The actin cytoskeleton was stained with fluorophore-conjugated phalloidin, and DNA was stained with DAPI. (E) The N-terminal region of LUZP1 is not required for the protein’s localization. RPE-1 stable cells stably expressing GFP-tagged LUZP1, full-length and the two truncation mutants represented in A were stained for GFP, glutamylated tubulin (centriole and cilium marker), and PCNT (a centrosome marker; left panels) or ARL13B (right panels).