Figure S4. mt DNA segregation and... | Rockefeller University Press

Figure S4.

$mtDNA segregation and replication are required for selective inheritance. (A) Representative images of the anterior end of regions 1 and 2A in a Drp1 knockdown germarium (driven by bam-gal4) labeled with TFAM-GFP and ATPs. An object with continuous ATPs staining was defined as a single mitochondrion (white dashed lines). Two adjacent but distinct ATPs puncta were also called as a single mitochondrion if they appeared in the same contour and were connected with one nucleoid (magenta dashed lines). Scale bar, 2 µm. (B) The number of nucleoids per mitochondrion was determined by counting the number of TFAM-GFP puncta within a single mitochondrion shown in A in regions 1 (GSCs or cystoblasts) and 2A from Drp1 knockdown ovaries (n = 5 germaria). The fraction of each group is shown. (C) Knockdown of Drp1 using bam-gal4 driver diminished the selection against the ts mtDNA in heteroplasmic mt:CoIT300I Dm (n = 10 for each genotype). Data presented are means ± SD. P < 0.001. (D) Knockdown of tamas using nanos-gal4 driver diminished the selection against the ts mtDNA in heteroplasmic mt:CoIT300I Dm (n = 10 for each genotype). Data presented are means ± SD. P < 0.001.$

mt DNA segregation and replication are required for selective inheritance. (A) Representative images of the anterior end of regions 1 and 2A in a Drp1 knockdown germarium (driven by bam-gal4) labeled with TFAM-GFP and ATPs. An object with continuous ATPs staining was defined as a single mitochondrion (white dashed lines). Two adjacent but distinct ATPs puncta were also called as a single mitochondrion if they appeared in the same contour and were connected with one nucleoid (magenta dashed lines). Scale bar, 2 µm. (B) The number of nucleoids per mitochondrion was determined by counting the number of TFAM-GFP puncta within a single mitochondrion shown in A in regions 1 (GSCs or cystoblasts) and 2A from Drp1 knockdown ovaries (n = 5 germaria). The fraction of each group is shown. (C) Knockdown of Drp1 using bam-gal4 driver diminished the selection against the ts mtDNA in heteroplasmic mt:CoI^T300I Dm (n = 10 for each genotype). Data presented are means ± SD. P < 0.001. (D) Knockdown of tamas using nanos-gal4 driver diminished the selection against the ts mtDNA in heteroplasmic mt:CoI^T300I Dm (n = 10 for each genotype). Data presented are means ± SD. P < 0.001.