Figure S2.
Expression pattern of germline-specific drivers and FISH assay. (A) Expression pattern of mitochondrially targeted GFP (mitoGFP) driven by bam-gal4 and nanos-gal4 drivers. Note the high expression level of mitoGFP in dividing cysts driven by bam-gal4. Ovaries are costained with DAPI (blue). Scale bar, 20 µm. (B–E) smFISH assay showing the knockdown efficiency of several RNAi lines. The images are representative images of smFISH using DNA probes targeted to Fis1 (B), Drp1 (C), cox5A (D), and mtSSB (E) in ovaries. Brackets in B and C indicate region 2A. Scale bar, 10 µm. (F) Quantification of the smFISH fluorescence intensity in germline cysts using region 2B follicle cells in the same germarium as the internal control (n = 4 for each genotype). The ratio of the fluorescence intensity in germline cysts to follicle cells are shown. Data presented are means ± SD. **, P < 0.05; ***, P < 0.001.

Expression pattern of germline-specific drivers and FISH assay. (A) Expression pattern of mitochondrially targeted GFP (mitoGFP) driven by bam-gal4 and nanos-gal4 drivers. Note the high expression level of mitoGFP in dividing cysts driven by bam-gal4. Ovaries are costained with DAPI (blue). Scale bar, 20 µm. (B–E) smFISH assay showing the knockdown efficiency of several RNAi lines. The images are representative images of smFISH using DNA probes targeted to Fis1 (B), Drp1 (C), cox5A (D), and mtSSB (E) in ovaries. Brackets in B and C indicate region 2A. Scale bar, 10 µm. (F) Quantification of the smFISH fluorescence intensity in germline cysts using region 2B follicle cells in the same germarium as the internal control (n = 4 for each genotype). The ratio of the fluorescence intensity in germline cysts to follicle cells are shown. Data presented are means ± SD. **, P < 0.05; ***, P < 0.001.

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