Crb-intra activates PM-targeted aPKC/Par-6 complex. (A) In HEK293 cells expressing either PB1^Par-6::iRFP or Par-6ΔPDZ::iRFP, PKCζ::RFP was strongly PM-localized, whereas Lgl::GFP was strongly reduced from PM. (B) In HEK293 cells expressing Lgl::GFP, PKCζ::RFP, and Par-6::iRFP, PM localization of Lgl::GFP was strongly reduced when BFP::Crb-intra, but not BFP::Crb-intra^ΔERLI or BFP::Cdc42^CA, was coexpressed. Lgl^S6A::GFP remained on PM in cells expressing PKCζ::RFP, Par-6::iRFP, and BFP::Crb-intra. Lgl::GFP remained on PM in cells expressing BFP::Crb-intra, Par-6::iRFP, and kinase-dead PKCζ^K281W::RFP. Scale bars: 5 µm.