Interaction between Par-6 and Crb-intra in HEK293 cells. (A) Par-6::iRFP localized to PM in HEK293 cells expressing BFP::Crb-intra, but not in cells expressing BFP::Crb-intra^ΔERLI. PKCζ^KR8Q::RFP was PM-localized in cells expressing Par-6::iRFP and BFP::Crb-intra but was cytosolic in cells expressing Par-6::iRFP and BFP::Crb-intra^ΔERLI. (B) Lgl::GFP was predominantly PM-localized in polarized MDCK cells overexpressing PKCζ::RFP or PKCζ::RFP^-2A-Par-6::iRFP. Lgl^S6A::GFP was PM-localized in MDCK cells expressing PKCζ::RFP^–2A-Par-6::iRFP. Scale bars: 5 µm.