Par-6::GFP expands to basolateral PM in Drosophila follicular epithelial cells under the acute loss of PIP₂. Cells overexpressing mRFP-FKBP-INPP5E and PM-bound Lck-FRB::CFP (not depicted) are labeled by nuclear RFP (asterisks in GFP images). Rapamycin (rapa) treatment induced strong PM localization of mRFP-FKBP-INPP5E, but Par-6::GFP remained largely on apical PM (A, cross-section view) with expansion to lateral PM (B, tangential view of basolateral PM). Scale bars: 5 µm.