PM targeting of PKCζ in HEK293 cells is independent of Cdc42. (A) PKCζ::GFP was cytosolic when coexpressed with BFP::Cdc42^CA. Par-6::RFP localized to PM in cells coexpressing GFP::Cdc42^CA. Coexpressed PKCζ and Pra-6 were PM-localized in cells expressing either BFP::Cdc42^CA or BFP::Cdc42^DN. Nonpolybasic PKCζ^KR8Q::GFP was PM-localized in cells expressing both BFP::Cdc42^CA and Par-6::RFP. (B) Overexpression of Cdc42^CA did not change DaPKC::GFP and DaPKC^KR8Q::GFP subcellular localization in Drosophila follicular epithelial cells. (C) DaPKC::GFP formed intracellular puncta (arrowheads) in cdc42-RNAi follicular cells. In B and C, cells expressing UAS-cdc42-RNAi or UAS-Cdc42^CA are marked by RFP expression. Asterisks highlight wild-type cells. Scale bars: 5 µm (A and B); 15 µm (C).