Par-6–dependent PM targeting of PKCζ in MCF7, COS7, and polarized MDCK cells. (A and B) Representative images showing that in MCF7 and COS7 cells, PKCζ and Par-6 were cytosolic when expressed alone, but both became PM-localized when coexpressed. (C) Representative images showing that in polarized MDCK cells, overexpressed PKCζ::GFP was cytosolic and Par-6::RFP was partially PM-localized. Both PKCζ::GFP and Par-6::RFP were PM-localized when coexpressed, whereas both PKCζ^KR8Q::GFP and Par-6::RFP were cytosolic when coexpressed. Scale bars: 5 µm.