Live dynamics of FA assembly and disassembly together with Rab18. (a) Live-cell imaging of U2OS cells cotransfected with GFP-Rab18 and RFP-vinculin. Cells were imaged every minute with a spinning disk confocal microscope. Magnifications of the boxed areas are shown in the lower panels and illustrate an example of FA disassembly (1) or assembly (2). Scale bars: top, 10 µm; bottom, 1 µm. (b and c) Fluorescence intensity profiles over time for GFP-Rab18 and RFP-vinculin at FAs. Fluorescence intensity profiles were individually normalized between 0 and 100 and then averaged. The graphs represent the mean intensity profiles ± SEM from four independent live-cell imaging experiments (n = 10).