Rab18-KNT1 interaction regulates FA size. (a) U2OS cells silenced with siRNA control, siRNA against Rab18, or silenced with siRNA against Rab18 and subsequently transfected with GFP-Rab18 were fixed and stained with Hoechst, rhodamine-conjugated phalloidin, and an antibody against vinculin. Scale bar: 10 µm. (b–d) Quantification of the size (b) and number of FAs per cell (c) or per 100 µm² cell area (d). The graphs represent the mean ± SEM for three independent experiments (n > 120 cells) *, P < 0.05; **, P < 0.01; ***, P < 0.001. (e) U2OS cells stably transfected with GFP-KNT1 FL, K1, or Δ201–445 were fixed and stained with DAPI, rhodamine-conjugated phalloidin, and an antibody against vinculin. Scale bar: 10 µm. (f–h) Quantification of the size (f) and number of FAs per cell (g) or per 100 µm² cell area (h). The graphs represent the mean ± SEM for three independent experiments (n > 90 cells). *, P < 0.05.