Knockdown of Rab18 delays the formation of ER–FA contacts. (a) Live imaging of U2OS cells treated with control siRNA or siRab18 and transfected with with RFP-vinculin and either ER-GFP or GFP-Rab18 as indicated. Cells were plated on fibronectin-coated dishes and imaged every 15 min with a spinning disk confocal microscope. Scale bar: 10 µm; insets: 2 µm. (b) Quantification of the time required for the formation of ER–FA contacts. The graph represents the mean ± SEM for at least three experiments (n > 20). *, P < 0.05; **, P < 0.01. (c) Quantification of the percentage of FAs in contact with the ER. The graph represents the mean ± SEM for at least three experiments (n > 20). *, P < 0.05; **, P < 0.01.