MLF2 is not required for bleb formation. (A) The relative amount of MLF2 mRNA transcript following a 50 nM siRNA treatment was analyzed by RT-qPCR. Relative mRNA levels were normalized to the siNT control. (B) The percentage of 4TorKO cells with K48-Ub foci upon treatment with 50 nM siNT or siMLF2. The values are means of three independent replicates (n = 3), and error bars indicate ±SD. (C) Amino acid sequence of human MLF2 with the predicted secondary structure using PHYRE2. Methionine and arginine residues are highlighted in yellow and red, respectively.