Figure 3.
MLF2 is enriched in nuclear herniations.(A) Torsin-deficient cells were transfected with MLF2-GFP and analyzed by immunoelectron microscopy to visualize the subcellular localization of the GFP fusion proteins. Scale bar, 2 µm; inset, 500 nm. N, nucleus; C, cytoplasm; asterisk, NPC. (B and C) EM images showing MLF2-GFP enrichment at sites of increased membrane curvature on the INM. Scale bars, 500 nm. Black arrow, ONM; white arrow, INM; N, nucleus; C, cytoplasm. (D) Percentage of cells showing MLF2-HA foci and K48-Ub foci from an asynchronous population, cells at the G1 to S phase transition (T = 0 h), and cells emerging from mitosis in early G1 (T = 12 h). Each result is the average of three independent experiments with 75 cells each. Error bars indicate ±SD. P value was calculated using a two-tailed t test.

MLF2 is enriched in nuclear herniations. (A) Torsin-deficient cells were transfected with MLF2-GFP and analyzed by immunoelectron microscopy to visualize the subcellular localization of the GFP fusion proteins. Scale bar, 2 µm; inset, 500 nm. N, nucleus; C, cytoplasm; asterisk, NPC. (B and C) EM images showing MLF2-GFP enrichment at sites of increased membrane curvature on the INM. Scale bars, 500 nm. Black arrow, ONM; white arrow, INM; N, nucleus; C, cytoplasm. (D) Percentage of cells showing MLF2-HA foci and K48-Ub foci from an asynchronous population, cells at the G1 to S phase transition (T = 0 h), and cells emerging from mitosis in early G1 (T = 12 h). Each result is the average of three independent experiments with 75 cells each. Error bars indicate ±SD. P value was calculated using a two-tailed t test.

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