Figure 5.
MAD2 recruitment to kinetochores is delayed when MAD1 cannot bind cyclin B1.(A) Maximum intensity projections at the indicated times from time-lapse fluorescence of parental RPE cyclin B1-Venus+/−:Ruby-MAD2+/−:RFP670-MIS12+/+ cells and the MAD1 E53/E56K+/+: RFP670-MIS12+/+ clones 7D2 and 8B12 showing Ruby-MAD2 localization relative to NEBD. Scale bar, 5 µm. (B) Quantification of Ruby-MAD2 colocalization with RFP670-MIS12 using the Coloc-3DT program (see Materials and methods) relative to NEBD. Graphs show values obtained from at least 40 cells for each clone in three independent experiments. Error bars indicate SEM.

MAD2 recruitment to kinetochores is delayed when MAD1 cannot bind cyclin B1. (A) Maximum intensity projections at the indicated times from time-lapse fluorescence of parental RPE cyclin B1-Venus+/−:Ruby-MAD2+/−:RFP670-MIS12+/+ cells and the MAD1 E53/E56K+/+: RFP670-MIS12+/+ clones 7D2 and 8B12 showing Ruby-MAD2 localization relative to NEBD. Scale bar, 5 µm. (B) Quantification of Ruby-MAD2 colocalization with RFP670-MIS12 using the Coloc-3DT program (see Materials and methods) relative to NEBD. Graphs show values obtained from at least 40 cells for each clone in three independent experiments. Error bars indicate SEM.

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