AnkG regulates the numbers of NF186-positive transport vesicles. (A and B) Quantification of vesicle numbers in control versus AnkG knockdown cultures in the anterograde (A) and retrograde (B) directions is shown. The total number of axons analyzed were NF186 with sh1AnkG, n = 22, sh2AnkG, n = 28, and scr, n = 27; and Necl1 with sh1AnkG, n = 6, sh2AnkG, n = 5, and scr, n = 5. Statistical significance was determined by two-tailed Student’s t test for unpaired data, with asterisks indicating P values of ****, P < 0.001; **, P < 0.01; and *, P < 0.05; n.s., not significant.