Figure 3.
Mutations that render portions of the VPS131-1390 channel unable to bind lipids (Fig. 2, B and C) abrogate VPS13 function in sporulation.(A) Low copy (CEN/pRS413) or multicopy (2-µm/pRS423) plasmids encoding GFP-tagged VPS13 constructs were expressed in vps13Δ strains and visualized as fluorescent punctae by fluorescence microscopy. See also Fig. S1. (B) Yeast lacking VPS13 fail to sporulate (Enyenihi and Saunders, 2003). Sporulation can be rescued by VPS13 but not the channel mutants. The experiment was repeated in triplicate. SD indicated by error bars.

Mutations that render portions of the VPS131-1390 channel unable to bind lipids ( Fig. 2, B and C ) abrogate VPS13 function in sporulation. (A) Low copy (CEN/pRS413) or multicopy (2-µm/pRS423) plasmids encoding GFP-tagged VPS13 constructs were expressed in vps13Δ strains and visualized as fluorescent punctae by fluorescence microscopy. See also Fig. S1. (B) Yeast lacking VPS13 fail to sporulate (Enyenihi and Saunders, 2003). Sporulation can be rescued by VPS13 but not the channel mutants. The experiment was repeated in triplicate. SD indicated by error bars.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal