Mitochondrial calcium uptake after PM injury in DRP1 KO and MiD49 patient cells. (A) Plot showing mCAR-GECO1 intensity after PM injury in WT (n = 12) and DRP1 KO MEFs (n = 14 cells). Calcium uptake was quantified over the entire cell, including both injury proximal and distal mitochondria. (B–E) Quantification of mitochondrial calcium uptake in WT and DRP1 KO cells after injury showing peak mitochondrial calcium (B), time to reach peak mitochondrial calcium (C), time for mitochondrial calcium to decrease to half peak (D), and area under the curve (E), illustrating mitochondrial calcium load from time of injury until calcium returned to baseline (n = 12 WT and 14 DRP1 KO cells for each). (F) Peak calcium uptake in injury-proximal and distal mitochondria of MiD49 patient cells (n = 6 cells). (G) Peak calcium uptake in injury-proximal mitochondria from healthy control and MiD49 patient cells (n = 9 control and 6 patient cells). (H) Quantification of time for calcium to reach half peak in control and MiD49 patient cells (n = 9 control and 6 patient cells). *, P < 0.05 by unpaired t test. #, P < 0.05 by Mann–Whitney U test. Error bars represent SEM.