Figure 3.
DRP1 facilitates injury-triggered mitochondrial fragmentation.(A) DRP1 recruitment to mitochondria in response to PM injury. Mitochondria are labeled by OMM protein TOM20, and dotted line indicates cell boundary. (B) Response of MitoTracker Green–labeled mitochondria (arrow) in DRP1 KO MEFs to PM injury. (C) Plot quantifying mitochondrial fragmentation after injury in WT (n = 16 cells) DRP1 KO cells (n = 27 cells). (D) FM 1-43 dye intensity after injury of WT (n = 56 cells) and DRP1 KO MEFs (n = 43 cells). (E) Plot showing percentage of cells that failed to repair (n = 6 experiments). Arrows mark the site of injury. Scale bar = 10 µm (A) and 5 µm (B). *, P < 0.05 by unpaired t test. #, P < 0.05 by Mann–Whitney U test. Error bars represent SEM.

DRP1 facilitates injury-triggered mitochondrial fragmentation. (A) DRP1 recruitment to mitochondria in response to PM injury. Mitochondria are labeled by OMM protein TOM20, and dotted line indicates cell boundary. (B) Response of MitoTracker Green–labeled mitochondria (arrow) in DRP1 KO MEFs to PM injury. (C) Plot quantifying mitochondrial fragmentation after injury in WT (n = 16 cells) DRP1 KO cells (n = 27 cells). (D) FM 1-43 dye intensity after injury of WT (n = 56 cells) and DRP1 KO MEFs (n = 43 cells). (E) Plot showing percentage of cells that failed to repair (n = 6 experiments). Arrows mark the site of injury. Scale bar = 10 µm (A) and 5 µm (B). *, P < 0.05 by unpaired t test. #, P < 0.05 by Mann–Whitney U test. Error bars represent SEM.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal