Mitochondrial morphology after injury and genetic KO of fission regulators MiD49 and Drp1. (A) Images showing WT MEF injured by glass bead. Injured cells are marked by presence of TRITC-dextran (red). Mitochondria are marked by MitoTracker Green (green). Magnified regions show area of mitochondrial fragmentation near injury site (Region 1) and area containing tubular mitochondria distal to injury site (Region 2). (B) Images showing mitochondria in healthy control and MiD49 patient fibroblasts. (C) Images showing mitochondrial shape in WT and DRP1 KO MEFs. In all cases, mitochondria are marked by MitoTracker Green. Scale bar = 10 µm or 5 µm (A, zoom).