ESCRT-III components form a plaque adjacent to the meiotic spindle, but are not required for nuclear closure. (A) Schematic of C. elegans oocyte meiosis II. (B and C) Fixed overview and magnified images of C. elegans oocytes expressing GFP::LEM-2 immunostained for GFP and CHMP-7 (B) or VPS-32 (C). LEM-2 examples in C are not scaled to the same intensity. Arrowheads mark plaque. Percentage of oocyte-derived pronuclei in which VPS-32 is localized to plaque marked by GFP::LEM-2 is shown. (D) Confocal images of embryo and of time lapse series of oocyte meiosis II with indicated markers. Arrowheads mark LEM-2 plaque. (E) Confocal image of C. elegans oocyte meiosis II with indicated markers. Below, magnified images of GFP::LEM-2. (F) Confocal images from time series of oocyte meiosis II with indicated markers. Arrowheads mark LEM-2 plaque. Below, line scans measuring background-corrected fluorescent intensities in arbitrary units (A.U.). (G) Schematic stereotypical nuclear events in early embryos relative to regression of the pseudocleavage, used as a reference time point 0 s (Penfield et al., 2018). Plot of nuclear:cytoplasmic ratio of GFP::NLS-LacI for the indicated conditions. A.U., arbitrary units. Average ± SEM is shown. n = no. of embryos. Scale bars, 5 µm.