Figure S4.
SKAP controls for stripping assay and coiled-coil alignments for CENP-FΔ1882-2292.(a) Immunofluorescence microscopy images of HeLa-K cells treated with control or DHC siRNA, arrested in 100 µM monastrol for 4 h, and stained with antibodies against CENP-C, α-tubulin, and SKAP. Scale bar, 5 µm. (b) Quantification of kinetochore SKAP signal relative to CENP-C in HeLa-K cells treated with control or DHC siRNA and arrested in 100 µM monastrol for 4 h. (c) Immunofluorescence microscopy images of HeLa-K, CENP-F-Mut1, and CENP-F-Mut2 cells treated with control or DIC siRNA, arrested in 100 µM monastrol for 4 h, and stained with antibodies against CENP-C, α-tubulin, and SKAP. Scale bar, 5 µm. (d) Quantification of kinetochore SKAP signal relative to CENP-C in HeLa-K, CENP-F-Mut1, and CENP-F-Mut2 cells treated with control or DIC siRNA and arrested in 100 µM monastrol for 4 h. (e) COILS (https://embnet.vital-it.ch/software/COILS_form.html) coiled-coil prediction for CENP-F and CENP-FΔ1882–2292. (f) COILS coiled-coil prediction for CENP-F^mEmerald and CENP-FΔ1882–2292^mEmerald. In b and d, boxes depict the median and first and third quartiles, and whiskers represent Q1 and Q3 ± 1.5× interquartile range.

SKAP controls for stripping assay and coiled-coil alignments for CENP-FΔ1882-2292 . (a) Immunofluorescence microscopy images of HeLa-K cells treated with control or DHC siRNA, arrested in 100 µM monastrol for 4 h, and stained with antibodies against CENP-C, α-tubulin, and SKAP. Scale bar, 5 µm. (b) Quantification of kinetochore SKAP signal relative to CENP-C in HeLa-K cells treated with control or DHC siRNA and arrested in 100 µM monastrol for 4 h. (c) Immunofluorescence microscopy images of HeLa-K, CENP-F-Mut1, and CENP-F-Mut2 cells treated with control or DIC siRNA, arrested in 100 µM monastrol for 4 h, and stained with antibodies against CENP-C, α-tubulin, and SKAP. Scale bar, 5 µm. (d) Quantification of kinetochore SKAP signal relative to CENP-C in HeLa-K, CENP-F-Mut1, and CENP-F-Mut2 cells treated with control or DIC siRNA and arrested in 100 µM monastrol for 4 h. (e) COILS (https://embnet.vital-it.ch/software/COILS_form.html) coiled-coil prediction for CENP-F and CENP-FΔ1882–2292. (f) COILS coiled-coil prediction for CENP-F^mEmerald and CENP-FΔ1882–2292^mEmerald. In b and d, boxes depict the median and first and third quartiles, and whiskers represent Q1 and Q3 ± 1.5× interquartile range.

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