Padi2 is required for proper regeneration and leukocyte recruitment. (A) Schematic of regeneration assay. Tail transections were performed through the notochord (red dotted line) at 2.5 dpf. Fin lengths were measured from the blood circulation to the end of the fin (blue solid line). (B) Representative bright field images of regeneration at 3 dpw and quantification of regenerate fin length from four independent replicates with n = 90 +/+, 95 −/−. (C) Representative images of 5 dpf developmental fins and quantification of developmental fin length from five independent replicates with n = 109 +/+, 108 −/−. (D) Schematic of leukocyte quantification region (in blue). (E) Representative images of leukocytes at a wound at 6, 24, and 48 hpw visualized with mCherry-labeled neutrophil nuclei (Tg(lyzC:H2B-mCherry)) and GFP-labeled macrophage nuclei (Tg(mpeg1:H2B-GFP)). Fluorescence image on the right, merge with bright-field on the left. Macrophage localization to the periphery of the notochord bead indicated with an arrow. (F) Quantification of neutrophil nuclei at a wound from three independent replicates (6 hpw, n = 62 +/+, 57 −/−; 24 hpw, n = 50 +/+, 47 −/−; 48 hpw, n = 63 +/+, n = 65 −/−). (G) Quantification of macrophage nuclei at a wound from three independent replicates (6 hpw, n = 61 +/+, 55 −/−; 24 hpw, n = 48 +/+, 44 −/−; and 48 hpw, n = 63 +/+, 57 −/−). All quantifications have lsmeans (± SEM) reported with P values calculated by ANOVA. Scale bars, 100 µm.