Spontaneous recovery from induced repositioning reveals transport dynamics of different organelles. (A) Assay. (B–J) Live-cell imaging (B, E, and H) and quantifications (C, D, F, G, I, and J) of LAMP1-mCherry-iLID (B–D), iLID-mCherry-RAB5 (E–G), or iLID-mCherry-RAB11 (H–J) in U2OS cells expressing opto-kinesin KIF1A(1–365)-VVDfast-GFP-SSPB(micro) or SSPB(micro)-GFP-GCN4-ppKin14-VIb before, during (middle), or 800 s after 200 s of illumination with blue light. Quantifications show change in peripheral organelle enrichment index (C, F, and I) or change in perinuclear enrichment index (D, G, and J). Graphs represent mean and SEM of at least three cells. Blue box indicates 470-nm illumination. Scale bars are 10 µm.