Dual color live-cell imaging of moss kinesin-14 and iLID-mCherry-RAB5. Related to Fig. 5. (A) Live-cell imaging of iLID-mCherry-RAB5 and SSPB(micro)-mVenus–ppKin14-VIb in U2OS before and after 10 min of illumination (see also Video 7). Scale bar is 10 µm. (B) Kymograph showing early endosome motility and induced colocalization with ppKin14-VIb as in A. (C) Example of induced early endosome motility (marked by arrowheads) as in A, before and immediately after illumination. Scale bar is 5 µm.